Recombinant Mouse Sema4d Protein, C-His-tagged
Product Description
Cat
IMP-4930
Official Symbol
Sema4d
Product Overview
Recombinant mouse SEMA4D (NP_038688.2) extracellular domain (Met 1-Arg 733) was expressed, with a C-terminal polyhistidine tag.
Description
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
Expression System
HEK293
Species
Mouse
Tag
C-His
Predicted N Terminal
Phe 24
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant mouse SEMA4D comprises 721 amino acids and has a calculated molecular mass of 80.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Arg733
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 96 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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