Recombinant Mouse SEMA4D Protein (Phe24-Met711), C-mFc-tagged
Product Description
Cat
IMP-9827
Official Symbol
Sema4d
Product Overview
Recombinant Mouse Semaphorin 4D/CD100 protein (Phe24-Met711) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Enables identical protein binding activity; semaphorin receptor binding activity; and transmembrane signaling receptor activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase signaling; regulation of neuron projection development; and regulation of phosphate metabolic process. Is integral component of plasma membrane.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Phe24
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Mass
Predicted Molecular Mass:
103 kDa

SDS-PAGE: 105-135 kDa, under reducing conditions
Protein length
Phe24-Met711
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -70 centigrade under sterile conditions after opening.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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