Recombinant Mouse SEMA4D Protein (Phe24-Met711), C-mFc-tagged
Product Description
Cat
IMP-9827
Official Symbol
Sema4d
Product Overview
Recombinant Mouse Semaphorin 4D/CD100 protein (Phe24-Met711) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Enables identical protein binding activity; semaphorin receptor binding activity; and transmembrane signaling receptor activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase signaling; regulation of neuron projection development; and regulation of phosphate metabolic process. Is integral component of plasma membrane.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Phe24
Form
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Mass
Predicted Molecular Mass:
103 kDa

SDS-PAGE: 105-135 kDa, under reducing conditions
Protein length
Phe24-Met711
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -70 centigrade under sterile conditions after opening.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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