Recombinant Mouse SIGLEC15 Protein (Arg24-Tyr250), C-6×His-tagged

Product Description

Cat

IMP-9577

Official Symbol

Siglec15

Product Overview

Recombinant Mouse Siglec-15 protein (Arg24-Tyr250) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

Predicted to be involved in regulation of actin cytoskeleton organization; regulation of bone resorption; and regulation of osteoclast development. Predicted to be integral component of membrane. Predicted to be part of protein-containing complex. Predicted to be active in plasma membrane.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Arg24

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 25 kDa
SDS-PAGE: 30-45 kDa, under reducing conditions

Protein length

Arg24-Tyr250

Bio-activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.075-0.75 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.075-0.75 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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