Recombinant Mouse Siglecg Protein (Met19-Lys543), C-mFc-tagged
Product Description
Cat
IMP-9562
Official Symbol
Siglecg
Product Overview
Recombinant Mouse Siglec-G protein (Met19-Lys543) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Predicted to enable SH2 domain binding activity; phosphatase binding activity; and sialic acid binding activity. Involved in several processes, including negative regulation of calcium-mediated signaling; negative regulation of inflammatory response to wounding; and regulation of B cell proliferation. Acts upstream of or within hematopoietic progenitor cell differentiation. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in thymus primordium. Orthologous to several human genes including SIGLEC10 (sialic acid binding Ig like lectin 10).
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Met19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 86 kDa
SDS-PAGE: 102-114 kDa, under reducing conditions
Protein length
Met19-Lys543
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-G Fc Chimera is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-G Fc Chimera is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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