Recombinant Mouse Siglecg Protein (Met19-Lys543), C-mFc-tagged

Product Description

Cat

IMP-9562

Official Symbol

Siglecg

Product Overview

Recombinant Mouse Siglec-G protein (Met19-Lys543) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Predicted to enable SH2 domain binding activity; phosphatase binding activity; and sialic acid binding activity. Involved in several processes, including negative regulation of calcium-mediated signaling; negative regulation of inflammatory response to wounding; and regulation of B cell proliferation. Acts upstream of or within hematopoietic progenitor cell differentiation. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in thymus primordium. Orthologous to several human genes including SIGLEC10 (sialic acid binding Ig like lectin 10).

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Met19

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 86 kDa
SDS-PAGE: 102-114 kDa, under reducing conditions

Protein length

Met19-Lys543

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-G Fc Chimera is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-G Fc Chimera is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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