Recombinant Mouse Sirpa protein, C-mFc Tag
Product Description
Cat
IMP-161
Official Symbol
Sirpa
Product Overview
Recombinant Mouse Sirpa protein(AA Lys 32-Asn 373)(Accession # BAA13520.1 (G365D)) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.
Expression System
HEK293
Species
Mouse
Tag
C-mFc Tag
Form
Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Lys 32-Asn 373
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Mouse SIRP alpha, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Mouse CD47, Fc Tag with a linear range of 8-63 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Human CD47 at 10 ug/mL can bind Mouse SIRP alpha, Mouse IgG2a Fc Tag with a linear range of 0.02-0.313 ug/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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