Recombinant Mouse Tgfbr1 Protein (Ala21-Glu121 & Thr22-Glu121), C-Fc-tagged
Product Description
Cat
IMP-9845
Official Symbol
Tgfbr1
Product Overview
Recombinant Mouse TGF-beta RI/ALK-5 protein, (Ala21-Glu121) & (Thr22-Glu121), with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.
Description
This gene encodes a member of the transforming growth factor beta (TGF-beta) receptor family of proteins. These proteins comprise one component of the TGF-beta signaling pathway, which transduces extracellular signals into gene expression changes to regulate a wide range of cellular responses, including proliferation, migration, differentiation and apoptosis. Homozygous knockout mice for this gene exhibit impaired angiogenesis and embryonic lethality. Alternative splicing results in multiple transcript variants.
Expression System
Sf 21 cells
Species
Mouse
Tag
C-Fc
Predicted N Terminal
Ala21 & Thr22
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 37.5 kDa (monomer)
SDS-PAGE: 40-50 kDa, reducing conditions
Protein length
Ala21-Glu121 & Thr22-Glu121
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Endoglin/CD105 Fc Chimera or Recombinant Mouse Endoglin/CD105 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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