Recombinant Mouse THBS1 Protein (Asn19-Ser1171), C-6×His-tagged

Product Description

Cat

IMP-9898

Official Symbol

Thbs1

Product Overview

Recombinant Mouse Thrombospondin-1 protein (Asn19-Ser1171) with a 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Starts at Asn19

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 129 kDa
SDS-PAGE: 130-155 kDa, reducing conditions

Protein length

Asn19-Ser1171

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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