Recombinant Mouse TIGIT Protein (Gly26-Thr143), C-mFc-tagged

Product Description

Cat

IMP-9881

Official Symbol

Tigit

Product Overview

Recombinant Mouse TIGIT protein (Gly26-Thr143) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Gly26

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 40.1 kDa (monomer)
SDS-PAGE: 50-60 kDa, reducing conditions

Protein length

Gly26-Thr143

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD155/PVR is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse TIGIT Fc Chimera that produces 50% optimal binding response is 10-50 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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