IMP-4989

Tlr3

Recombinant soluble form of mouse TLR3 (NP_569054.2) extracellular domain (Cys 29-Leu 705) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLR3/CD283 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA, a sign of viral infection. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses.

Insect cell

Mouse

C-His

Cys 29

Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.5, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The secreted recombinant mouse TLR3 consists of 688 amino acids and has a calculated molecular mass of 78 kDa. The recombinant protein migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Cys29-Leu705

< 1.0 EU/μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.