Recombinant Mouse Tnf Protein, No Tag

Product Description

Cat

IMP-4997

Official Symbol

Tnf

Product Overview

Recombinant soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the N-terminus.

Description

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Expression System

E. coli

Species

Mouse

Tag

No Tag

Predicted N Terminal

Met

Form

Lyophilized from sterile PBS, pH7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions.

Protein length

Leu80-Leu235

Endotoxin

Please contact us for more information.

Purity

> 98 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-Cell based assay 1

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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