Mouse Tnf (NP_038721, 80 a.a.-235 a.a.) partial recombinant protein expressed in E. coli.
Expression System
E. coli
Species
Mouse
Tag
Tag free
Form
Liquid, In PBS, pH 7.4
Molecular Mass
17.4 kDa
Protein length
80-235
Endotoxin
> 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE
Storage
Stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
SDS-PAGE
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Bioactivity-ELISA 1
Tnf (Mouse) Recombinant Protein stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cell. The ED50 range ≤ 0.06 ng/mL.