Recombinant Mouse TNFa protein, N-His Tag

Product Description

Cat

IMP-2292

Official Symbol

TNFa

Product Overview

Recombinant Mouse TNFa protein(P06804)(Leu80~Leu235), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Mouse

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

25kDa

Protein length

Met1~Gln210

Purity

> 95%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

Cell apoptosis of A549 cells after stimulated by TNFa

TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1. (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h; (B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h; (C)A549 cells cultured in DMEM for 48h.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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