Recombinant Mouse TNFa protein, N-His Tag

Product Description

Cat

IMP-2293

Official Symbol

TNFa

Product Overview

Recombinant Mouse TNFa protein(P06804)(Gly57~Leu235), fused with N-terminal His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Mouse

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

27kDa

Protein length

Met1~Gln210

Purity

> 95%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

(A)A549 cells cultured in DMEM,stimulated with 10ng/ml TNF-a for 72h; (B)Unstimulated A549 cells cultured in DMEM for 72h.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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