Recombinant Mouse TNFa protein, N-His Tag
Product Description
Cat
IMP-2293
Official Symbol
TNFa
Product Overview
Recombinant Mouse TNFa protein(P06804)(Gly57~Leu235), fused with N-terminal His Tag, was expressed in HEK293.
Expression System
HEK293
Species
Mouse
Tag
N-His Tag
Form
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass
27kDa
Protein length
Met1~Gln210
Purity
> 95%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Effect of TNF-a on A549 cells

(A)A549 cells cultured in DMEM,stimulated with 10ng/ml TNF-a for 72h; (B)Unstimulated A549 cells cultured in DMEM for 72h.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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