Recombinant Mouse Tnfrsf17 Protein, C-His&C-hFc-tagged
Product Description
Cat
IMP-1854
Official Symbol
Tnfrsf17
Product Overview
Recombinant extracellular domain of mouse BCMA (NP_035738.1) (Met 1-Thr 49) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description
Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.
Expression System
HEK293
Species
Mouse
Tag
C-His&C-hFc
Predicted N Terminal
Glu 20
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse BCMA/Fc chimera is a disulfide-linked homodimer The reduced monomer consists of 297 amino acids and has a predicted molecular mass of 33.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm BCMA/Fc monomer is approximately 35-48 kDa due to glycosylation.
Protein length
Met1-Thr49
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human BAFF at 10 μg/mL (100 μL/well) can bind mouse BCMA-Fch, The EC50 of mouse BCMA-Fch is 0.02-0.06 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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