Recombinant Mouse TNFRSF18 Protein (Ser22-His153), C-hFc and 6×His-tagged

Product Description

Cat

IMP-9828

Official Symbol

Tnfrsf18

Product Overview

Recombinant Mouse GITR/TNFRSF18 protein (Ser22-His153) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc and 6×His

Predicted N Terminal

Ser22

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 42 kDa (monomer)
SDS-PAGE: 55-65 kDa, reducing conditions

Protein length

Ser22-His153

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rmGITR/Fc Chimera at 2 μg/mL (100 μL/well) can bind rmGITR Ligand with a linear range of 0.8-50 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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