Recombinant Mouse TNFRSF1B Protein (Val23-Gly258), C-mFc-tagged
Product Description
Cat
IMP-9983
Official Symbol
Tnfrsf1b
Product Overview
Recombinant Mouse TNF RII/TNFRSF1B protein (Val23-Gly258) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Val23
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 52 kDa
SDS-PAGE: 66-76 kDa, reducing conditions
Protein length
Val23-Gly258
Bio-activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3-15 ng/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3-15 ng/mL

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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