Recombinant Mouse Tnfrsf1b Protein (Val23-Gly258), Tag Free
Product Description
Cat
IMP-9801
Official Symbol
Tnfrsf1b
Product Overview
Recombinant Mouse sTNF RII/TNFRSF1B protein (Val23-Gly258) without tag was expressed in E. coli.
Description
Enables tumor necrosis factor-activated receptor activity. Involved in several processes, including negative regulation of extracellular matrix constituent secretion; regulation of nervous system development; and semi-lunar valve development. Acts upstream of or within several processes, including RNA destabilization; apoptotic signaling pathway; and negative regulation of inflammatory response. Located in membrane raft. Is expressed in several structures, including blood; dorsal aorta; extraembryonic component; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; acne; bone disease (multiple); glomerulonephritis (multiple); and lung disease (multiple). Orthologous to human TNFRSF1B (TNF receptor superfamily member 1B).
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Val23
Form
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 25 kDa
Protein length
Val23-Gly258
Bio-activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1-3 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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