Recombinant Mouse Tnfrsf1b Protein (Val23-Gly258), Tag Free
Product Description
Cat
IMP-9801
Official Symbol
Tnfrsf1b
Product Overview
Recombinant Mouse sTNF RII/TNFRSF1B protein (Val23-Gly258) without tag was expressed in E. coli.
Description
Enables tumor necrosis factor-activated receptor activity. Involved in several processes, including negative regulation of extracellular matrix constituent secretion; regulation of nervous system development; and semi-lunar valve development. Acts upstream of or within several processes, including RNA destabilization; apoptotic signaling pathway; and negative regulation of inflammatory response. Located in membrane raft. Is expressed in several structures, including blood; dorsal aorta; extraembryonic component; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; acne; bone disease (multiple); glomerulonephritis (multiple); and lung disease (multiple). Orthologous to human TNFRSF1B (TNF receptor superfamily member 1B).
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Val23
Form
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 25 kDa
Protein length
Val23-Gly258
Bio-activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1-3 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info