Recombinant Mouse TNFRSF4 Protein (Val20-Pro211), C-hFc-tagged

Product Description

Cat

IMP-9665

Official Symbol

Tnfrsf4

Product Overview

Recombinant Mouse OX40/TNFRSF4 protein (Val20-Pro211) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Val20

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 48 kDa (monomer)
SDS-PAGE: 65-75 kDa, reducing conditions

Protein length

Val20-Pro211

Bio-activity

Measured by its ability to inhibit OX40 Ligand induced IL-2 secretion by mouse T cells. The ED50 for this effect is 0.1-0.3 μg/mL in the presence of 30 ng/mL of Recombinant Mouse OX40 Ligand/TNFSF4.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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