Recombinant Mouse Tnfrsf9 protein, C-Fc Tag

Product Description

Cat

IMP-235

Official Symbol

Tnfrsf9

Product Overview

Recombinant Mouse Tnfrsf9 protein(AA Val 24-Leu 211)(Accession # Q8R037-1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 46.7 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE).

Protein length

Val 24-Leu 211

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse 4-1BB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Mouse 4-1BB, Fc Tag at 2 ug/mL (100 uL/well) can bind Human 4-1BB Ligand, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 2

Immobilized Mouse 4-1BB, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human 4‑1BB Ligand, Fc Tag, Avi Tag with a linear range of 0.01-0.156 ug/mL.

Bioactivity-BLI 1

Loaded Mouse 4-1BB, Fc Tag on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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