Recombinant Mouse Tnfsf10 Protein (Pro118-Asn291), Tag Free
Product Description
Cat
IMP-9652
Official Symbol
Tnfsf10
Product Overview
Recombinant Mouse TRAIL/TNFSF10 protein (Pro118-Asn291) without tag was expressed in E. coli.
Description
Enables TRAIL binding activity and tumor necrosis factor receptor superfamily binding activity. Acts upstream of or within positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular space and plasma membrane. Predicted to be integral component of membrane. Is expressed in central nervous system; genitourinary system; gut; and retina. Human ortholog(s) of this gene implicated in arteriosclerosis and small cell carcinoma. Orthologous to human TNFSF10 (TNF superfamily member 10).
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Pro118
Form
Lyophilized from a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4 with Trehalose and with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 20 kda
Protein length
Pro118-Asn291
Bio-activity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.2-1.2 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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