Recombinant Mouse Tnfsf10 Protein (Pro118-Asn291), Tag Free

Product Description

Cat

IMP-9652

Official Symbol

Tnfsf10

Product Overview

Recombinant Mouse TRAIL/TNFSF10 protein (Pro118-Asn291) without tag was expressed in E. coli.

Description

Enables TRAIL binding activity and tumor necrosis factor receptor superfamily binding activity. Acts upstream of or within positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular space and plasma membrane. Predicted to be integral component of membrane. Is expressed in central nervous system; genitourinary system; gut; and retina. Human ortholog(s) of this gene implicated in arteriosclerosis and small cell carcinoma. Orthologous to human TNFSF10 (TNF superfamily member 10).

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Predicted N Terminal

Pro118

Form

Lyophilized from a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4 with Trehalose and with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 20 kda

Protein length

Pro118-Asn291

Bio-activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.2-1.2 ng/mL.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry