Recombinant Mouse TNFSF13B Protein (Ala127-Leu309)
Product Description
Cat
IMP-9933
Official Symbol
Tnfsf13b
Product Overview
Recombinant Mouse BAFF/BLyS/TNFSF13B protein (Ala127-Leu309) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Ala127
Form
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein..
Molecular Mass
Predicted Molecular Mass: 21 kDa
SDS-PAGE: 19-23 kDa (monomer) & 38-47 kDa (non-reducible dimer), reducing conditions
Protein length
Ala127-Leu309
Bio-activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-0.5 ng/mL in the presence of goat anti-mouse IgM μ chain.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Noncovalently-linked homotrimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100-500 μg/mL in sterile water.
Bioactivity-ELISA 1
Bioactivity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-0.5 ng/mL in the presence of goat anti-mouse IgM μ chain.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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