Recombinant Mouse TNFSF14 Protein (Asp72-Val239), N-10×His-tagged
Product Description
Cat
IMP-9699
Official Symbol
Tnfsf14
Product Overview
Recombinant Mouse LIGHT/TNFSF14 protein (Asp72-Val239) with a 10-His tag at N-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-10×His
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 21 kDa
SDS-PAGE: 24-34 kDa, reducing conditions
Protein length
Asp72-Val239
Bio-activity
Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 2-5 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody and recombinant human IFN-gamma.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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