IMP-386

Tnfsf14

Recombinant Mouse Tnfsf14 protein (NP_062291.1) (Asp72-Val239) was expressed in Baculovirus-Insect Cells, fused with a polyhistidine tag at the N-terminus.

Tnfsf14, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / Tnfsf14 / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / Tnfsf14 / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of Tnfsf14 in vivo. TNFSF14 / Tnfsf14 / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4 and CD T cells. In addition, TNFSF14 / Tnfsf14 / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / Tnfsf14 / CD258 is being actively investigated as a possible basis for cancer treatment.

Insect Cells

Mouse

N-His Tag

His

Lyophilized from sterile 20 mM Tris, pH 8.0, 500 mM NaCl, 10 % gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

The recombinant mouse TNFSF14 consists of 184 amino acids and predicts a molecular mass of 20.5 kDa.

Asp72-Val239

< 1.0 EU per μg protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE.

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.