Recombinant Mouse Tnfsf18 protein, N-Fc Tag
Product Description
Cat
IMP-181
Official Symbol
Tnfsf18
Product Overview
Recombinant Mouse Tnfsf18 protein(AA Thr 47-Ser 173)(Accession # AAO89011) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the N-terminus.
Expression System
HEK293
Species
Mouse
Tag
N-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.4 kDa. As a result of glycosylation, the protein migrates as 45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE)
Protein length
Thr 47-Ser 173
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Mouse GITR Ligand, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse GITR, His Tag at 5 ug/mL (100 uL/well) can bind Mouse GITR Ligand, Fc Tag with a linear range of 5-156 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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