Recombinant Mouse TNFSF18 Protein (Thr47-Ser173), N-10×His-tagged

Product Description

Cat

IMP-9720

Official Symbol

Tnfsf18

Product Overview

Recombinant Mouse GITR Ligand/TNFSF18 protein (Thr47-Ser173) with an N-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

N-10×His

Predicted N Terminal

His

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 16 kDa
SDS-PAGE: 16-26 kDa, reducing conditions

Protein length

Thr47-Ser173

Bio-activity

Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 0.02-0.3 μg/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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