Recombinant Mouse Tnfsf4 Protein, C-His-tagged

Product Description

Cat

IMP-1355

Official Symbol

Tnfsf4

Product Overview

Recombinant mouse TNFSF4 (NP_033478.1) (Ser51-Leu198) was expressed with a polyhistidine tag at the C-terminus.

Description

OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig-and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Expression System

HEK293

Species

Mouse

Tag

C-His

Predicted N Terminal

Ser 51

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant mouse TNFSF4 consists of 159 amino acids and predicts a molecular mass of 18.1 kDa.

Protein length

Ser51-Leu198

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized TNFSF4 Protein, Mouse, (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind OX40, Human, (His&hFc Tag), the EC50 of OX40-Fc2h is 30-90 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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