Recombinant Mouse Tnfsf4 Protein, C-His-tagged

Product Description

Cat

IMP-1355

Official Symbol

Tnfsf4

Product Overview

Recombinant mouse TNFSF4 (NP_033478.1) (Ser51-Leu198) was expressed with a polyhistidine tag at the C-terminus.

Description

OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig-and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Expression System

HEK293

Species

Mouse

Tag

C-His

Predicted N Terminal

Ser 51

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant mouse TNFSF4 consists of 159 amino acids and predicts a molecular mass of 18.1 kDa.

Protein length

Ser51-Leu198

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized TNFSF4 Protein, Mouse, (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind OX40, Human, (His&hFc Tag), the EC50 of OX40-Fc2h is 30-90 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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