Recombinant Mouse TNFSF9 Protein (Arg104-Glu309), N-10×His-tagged
Product Description
Cat
IMP-9664
Official Symbol
Tnfsf9
Product Overview
Recombinant Mouse 4-1BB Ligand/TNFSF9 protein (Arg104-Glu309) with a Met and 10-His tag at N-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-10×His
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 25.7 kDa
SDS-PAGE: 41-44 kDa, reducing conditions
Protein length
Arg104-Glu309
Bio-activity
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 μg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody. Measured by its ability to bind Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera in a functional ELISA.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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