Recombinant Mouse TNFSF9 Protein (Arg104-Glu309), N-10×His-tagged
Product Description
Cat
IMP-9664
Official Symbol
Tnfsf9
Product Overview
Recombinant Mouse 4-1BB Ligand/TNFSF9 protein (Arg104-Glu309) with a Met and 10-His tag at N-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-10×His
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 25.7 kDa
SDS-PAGE: 41-44 kDa, reducing conditions
Protein length
Arg104-Glu309
Bio-activity
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 μg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody. Measured by its ability to bind Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera in a functional ELISA.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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