Recombinant Mouse TSLP Protein (Tyr20-Glu140), C-10×His-tagged
Product Description
Cat
IMP-9834
Official Symbol
Tslp
Product Overview
Recombinant Mouse TSLP protein (Tyr20-Glu140) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-10×His
Predicted N Terminal
Tyr20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 15 kDa
SDS-PAGE: 22-29 kDa, reducing conditions
Protein length
Tyr20-Glu140
Bio-activity
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse IL-7 R alpha. The ED50 for this effect is 0.5-2.5 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info