Recombinant Mouse ULBP1 Protein (Pro26-Thr211), C-6×His-tagged

Product Description

Cat

IMP-9735

Official Symbol

Ulbp1

Product Overview

Recombinant Mouse ULBP-1/MULT-1 protein (Pro26-Thr211) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Pro26

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 22 kDa (monomer)
SDS-PAGE: 28-43 kDa, reducing conditions

Protein length

Pro26-Thr211

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse NKG2D Fc Chimera at 2 μg/mL (100 μL/well) can bind Recombinant Mouse ULBP-1/MULT-1 with a linear range of 1-50 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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