Recombinant Mouse Vegfd Protein, N-hFc-tagged

Product Description

Cat

IMP-5048

Official Symbol

Vegfd

Product Overview

Recombinant mature form of mouse FIGF (P97946) (Phe 98-Ser 206) was fused with the Fc region of human IgG1 at the N-terminus.

Description

Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), belongs to the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. FIGF protein is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. FIGF protein is secreted as a non-covelent homodimer in an antiparallel fashion. Human FIGF protein is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. FIGF protein is structurally and functionally similar to VEGF-C. Therefore, FIGF protein binds and activates VEGFR-2 (Flk1) and VEGFR-3 (Flt4) receptors, and may particularly be involved in cancers, such as breast cancer, epithelial ovarian carcinoma and so on.

Expression System

HEK293

Species

Mouse

Tag

N-hFc

Predicted N Terminal

Glu

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant mouse FIGF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 369 amino acids and has a calculated molecular mass of 40.6 kDa.The apparent molecular mass of the protein is approximately 45 and 36 kDa in SDS-PAGE under reducing conditions.

Protein length

Phe98-Ser206

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized human VEGFR3-His at 10 μg/mL (100 μL/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 49 ng/mL. Immobilized mouse VEGFR3-His at 10 μg/mL (100 μL/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 44 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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