Recombinant Mouse Vegfd Protein (Phe98-Ser206), C-6×His-tagged

Product Description

Cat

IMP-9819

Official Symbol

Vegfd

Product Overview

Recombinant Mouse VEGF-D protein (Phe98-Ser206) with a C-terminal 6×His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Description

This gene encodes a member of the vascular endothelial growth factor (VEGF) family that acts as a ligand for vascular endothelial growth factor receptor 3. Signalling through this protein is important for growth and development of endothelial and vascular tissue. Alternative splicing results in multiple transcript variants.

Expression System

Sf 21 cells

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Phe98

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 13 kDa
SDS-PAGE: 14-20 kDa, reducing conditions

Protein length

Phe98-Ser206

Bio-activity

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is 0.2-0.8 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rmFlt-4/Fc Chimera at 5 μg/mL (100 μL/well) can bind rmVEGF-D with an apparent KD < 50 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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