Recombinant Mouse ZNRF3 Protein (Lys53-Met216), C-mFc-tagged

Product Description

Cat

IMP-9914

Official Symbol

Znrf3

Product Overview

Recombinant Mouse ZNRF3 protein (Lys53-Met216) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Lys53

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 45 kDa
SDS-PAGE: 51-60 kDa, reducing conditions

Protein length

Lys53-Met216

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse R-Spondin 3 is coated at 0.25 μg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent Kd <0.2 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE with silver staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. When Recombinant Mouse R-Spondin 3 is coated at 0.25 μg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent Kd <0.2 nM.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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