Recombinant Pig KITLG protein, N-His Tag
Product Description
Cat
IMP-2729
Official Symbol
KITLG
Product Overview
Recombinant Pig KITLG protein(Thr25~Ala191), fused with N-terminal His Tag, was expressed in HEK293.
Expression System
HEK293
Species
Pig
Tag
N-His Tag
Form
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass
22kDa
Protein length
Thr47~Ser219
Purity
> 95%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Cell proliferation of TF-1 cells after stimulated with SCF

(A)TF-1 cells cultured in RPMI-1640, stimulted with 1ng/mL SCF for 72h. (B)Unstimulated TF-1 cells cultured in RPMI-1640 for 72h.

Bioactivity-ELISA 2
The dose-effect curve of SFC on TF-1 cells

The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 6.07 to 13.69ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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