Recombinant Rabbit TNF protein
Product Description
Cat
IMP-5953
Official Symbol
TNF
Product Overview
Recombinant Rabbit TNF protein(P04924)(77-235aa) was expressed in E.coli.
Expression System
E.coli
Species
Rabbit
Tag
N/A
Form
Lyophilized from a 0.2 μm filtered 20 mM PB, 300 mM NaCl, pH 7.4
Molecular Mass
17.59 kDa
Protein length
77-235aa
AA Sequence
MVTLRSASRALSDKPLAHVVANPQVEGQLQWLSQRANALLANGMKLTDNQLVVPADGLYLIYSQVLFSGQGCRSYVLLTHTVSRFAVSYPNKVNLLSAIKSPCHRETPEEAEPMAWYEPIYLGGVFQLEKGDRLSTEVNQPEYLDLAESGQVYFGIIAL
Endotoxin
Less than 1.0 EU/µg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
The ED50 as determined in a cytotoxicity assay using L‑929 mouse fibroblast cells is less than 20 pg/ml in the presence of the metabolic inhibitor actinomycin D.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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