Recombinant Rat CCN2 Protein (Gln25-Ala347)
Product Description
Cat
IMP-10228
Official Symbol
Ccn2
Product Overview
Recombinant Rat CTGF protein (Gln25-Ala347) without tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Expression System
HEK293
Species
Rat
Tag
Tag Free
Predicted N Terminal
No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Asp26
Form
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 35-41 kDa, reducing conditions
Protein length
Gln25-Ala347
Bio-activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1-5 μg/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in water.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1-5 μg/mL

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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