Recombinant Rat CCN2 Protein (Gln25-Ala347)
Product Description
Cat
IMP-10228
Official Symbol
Ccn2
Product Overview
Recombinant Rat CTGF protein (Gln25-Ala347) without tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Expression System
HEK293
Species
Rat
Tag
Tag Free
Predicted N Terminal
No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Asp26
Form
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 35-41 kDa, reducing conditions
Protein length
Gln25-Ala347
Bio-activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1-5 μg/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in water.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1-5 μg/mL

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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