Recombinant Rat CD27 Protein (Thr21-Arg183), C-mFc-tagged
Product Description
Cat
IMP-10283
Official Symbol
Cd27
Product Overview
Recombinant Rat CD27/TNFRSF7 protein (Thr21-Arg183) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD28-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-mFc
Predicted N Terminal
Thr21
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 45.1 kDa (monomer)
SDS-PAGE: 60-66 kDa, reducing conditions
Protein length
Thr21-Arg183
Bio-activity
Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.4-1.6 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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