Recombinant Rat Cd40 protein, C-His Tag
Product Description
Cat
IMP-376
Official Symbol
Cd40
Product Overview
Recombinant Rat Cd40 protein (NP_599187.1) (Met1-Arg193) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
Description
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Expression System
HEK293
Species
Rat
Tag
C-His Tag
Predicted N Terminal
Leu 20
Form
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass
The recombinant rat CD40 comprises 185 amino acids and predicts a molecular mass of 20.6 kDa. The apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein length
Met1-Arg193
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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