Recombinant Rat Cd40 protein, C-His Tag

Product Description

Cat

IMP-376

Official Symbol

Cd40

Product Overview

Recombinant Rat Cd40 protein (NP_599187.1) (Met1-Arg193) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Description

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Expression System

HEK293

Species

Rat

Tag

C-His Tag

Predicted N Terminal

Leu 20

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Molecular Mass

The recombinant rat CD40 comprises 185 amino acids and predicts a molecular mass of 20.6 kDa. The apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein length

Met1-Arg193

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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