Recombinant Rat CD40LG Protein (Met112-Leu260), N-HA-tagged

Product Description

Cat

IMP-10365

Official Symbol

Cd40lg

Product Overview

Recombinant Rat CD40 Ligand/TNFSF5 protein (Met112-Leu260) with a HA (YPYDVPDYA) tag at N-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Expression System

CHO

Species

Rat

Tag

N-HA

Predicted N Terminal

Tyr

Form

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 22 kDa
SDS-PAGE: 22-30 kDa, reducing conditions

Protein length

Met112-Leu260

Bio-activity

Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.15-0.9 ng/mL in the presence of 0.1 μg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE with silver staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in steril PBS containing at least 0.1% human or bovine serum albumin.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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