Recombinant Rat CD40LG Protein (Met112-Leu260), N-HA-tagged

Product Description

Cat

IMP-10365

Official Symbol

Cd40lg

Product Overview

Recombinant Rat CD40 Ligand/TNFSF5 protein (Met112-Leu260) with a HA (YPYDVPDYA) tag at N-terminus was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Expression System

CHO

Species

Rat

Tag

N-HA

Predicted N Terminal

Tyr

Form

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 22 kDa
SDS-PAGE: 22-30 kDa, reducing conditions

Protein length

Met112-Leu260

Bio-activity

Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.15-0.9 ng/mL in the presence of 0.1 μg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE with silver staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in steril PBS containing at least 0.1% human or bovine serum albumin.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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