Recombinant Rat Cd48 protein, C-His Tag
Product Description
Cat
IMP-361
Official Symbol
Cd48
Product Overview
Recombinant Rat Cd48 protein (NP_620803.1) (Met1-Arg216) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
Description
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Expression System
HEK293
Species
Rat
Tag
C-His Tag
Predicted N Terminal
Phe 23
Form
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass
The recombinant rat CD48 comprises 205 amino acids and predicts a molecular mass of 23.9 kDa. The apparent molecular mass of the recombinant protein is approximately 38-43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein length
Met1-Arg216
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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