Recombinant Rat CX3CL1 Protein (Leu22-Gly100)
Product Description
Cat
IMP-10236
Official Symbol
Cx3cl1
Product Overview
Recombinant Rat CX3CL1/Fractalkine protein (Leu22-Gly100) without tag was expressed in E. coli.
Description
This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections.
Expression System
E. coli
Species
Rat
Tag
Tag Free
Predicted N Terminal
Leu22
Form
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 9.0 kDa
Protein length
Leu22-Gly100
Bio-activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED50 for this effect is 15-75 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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