Recombinant Rat CX3CL1 protein, N-His Tag

Product Description

Cat

IMP-2754

Official Symbol

CX3CL1

Product Overview

Recombinant Rat CX3CL1 protein(O55145)(Gln83~Pro328), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Rat

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

27kDa

Protein length

Phe25~Thr211

Purity

> 95%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

The chemotactic effect of CX3CL1 on THP-1 cells

(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 31.25ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h; (B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.

Bioactivity-ELISA 2

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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