Recombinant Rat DKK1 Protein (Ser23-His270), C-6×His-tagged
Product Description
Cat
IMP-9794
Official Symbol
Dkk1
Product Overview
Recombinant Rat Dkk-1 protein (Ser23-His270) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-6×His
Predicted N Terminal
Ser23
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 27.6 kDa
SDS-PAGE: 38-42 kDa, reducing conditions
Protein length
Ser23-His270
Bio-activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Rat Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 50-300 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Rat Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 50-300 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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    1-516-512-3133
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