Recombinant Rat EPHB1 Protein (Met18-Gln538), C-hFc-tagged
Product Description
Cat
IMP-9692
Official Symbol
Ephb1
Product Overview
Recombinant Rat EphB1 protein (Met18-Gln538) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-hFc
Predicted N Terminal
Met18
Form
Lyophilized from a 0.2 μm filtered solution in Tris.
Molecular Mass
Predicted Molecular Mass: 85 kDa (monomer)
SDS-PAGE: 102 kDa, reducing conditions
Protein length
Met18-Gln538
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat EphB1 Fc Chimera at 2 μg/mL (100 μL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.31-20 ng/mL. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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