Recombinant Rat FCGR1A Protein (Gln16-Pro285), C-6×His-tagged
Product Description
Cat
IMP-9972
Official Symbol
Fcgr1a
Product Overview
Recombinant Rat Fc gamma RI/CD64 protein (Gln16-Pro285) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.
Expression System
Mouse myeloma cell line
Species
Rat
Tag
C-6×His
Predicted N Terminal
Thr21 observed&Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17
Form
Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Molecular Mass
Predicted Molecular Mass: 31 kDa
SDS-PAGE: 36-48 kDa, reducing conditions
Protein length
Gln16-Pro285
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 1 mg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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  • 1-631-559-9269
    1-516-512-3133
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