Recombinant Rat FCGR1A Protein (Gln16-Pro285), C-6×His-tagged

Product Description

Cat

IMP-9972

Official Symbol

Fcgr1a

Product Overview

Recombinant Rat Fc gamma RI/CD64 protein (Gln16-Pro285) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

C-6×His

Predicted N Terminal

Thr21 observed&Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17

Form

Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.

Molecular Mass

Predicted Molecular Mass: 31 kDa
SDS-PAGE: 36-48 kDa, reducing conditions

Protein length

Gln16-Pro285

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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