Recombinant Rat FCGR1A Protein (Gln16-Pro285), C-6×His-tagged

Product Description

Cat

IMP-9972

Official Symbol

Fcgr1a

Product Overview

Recombinant Rat Fc gamma RI/CD64 protein (Gln16-Pro285) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

C-6×His

Predicted N Terminal

Thr21 observed&Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17

Form

Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.

Molecular Mass

Predicted Molecular Mass: 31 kDa
SDS-PAGE: 36-48 kDa, reducing conditions

Protein length

Gln16-Pro285

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in PBS.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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