Recombinant Rat FCGR2A Protein (Asp37-His220), C-6×His-tagged
Product Description
Cat
IMP-9982
Official Symbol
Fcgr2a
Product Overview
Recombinant Rat Fc gamma RIIA/CD32a protein (Asp37-His220) with a C-terminal 6×His tag was expressed in Human embryonic kidney cell.
Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Rat
Tag
C-6×His
Predicted N Terminal
Asp37
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 22 kDa
SDS-PAGE: 29-44 kDa, reducing conditions
Protein length
Asp37-His220
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RIIA/CD32a is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 1.25-6.25 μg/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RIIA/CD32a is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 1.25-6.25 μg/mL

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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