Recombinant Rat ICOSLG Protein (Glu25-Lys261), C-hFc-tagged

Product Description

Cat

IMP-10419

Official Symbol

Icoslg

Product Overview

Recombinant Rat B7-H2 protein (Glu25-Lys261) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

C-hFc

Predicted N Terminal

Glu25

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 54 kDa
SDS-PAGE: 70-90 kDa, under reducing conditions

Protein length

Glu25-Lys261

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat B7-H2 Fc Chimera is Immobilized at 1.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICOS Fc Chimera that produces 50% optimal binding response is found to be approximately 10-100 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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