Recombinant Rat IL-17A (Ala18-Ser150, Ile46Leu) and IL-17F (Arg29-Ala161) Protein
Product Description
Cat
IMP-10523
Official Symbol
Il17a
Product Overview
Recombinant Rat IL-17A (Ala18-Ser150, Ile46Leu) and IL-17F (Arg29-Ala161) protein without tag was expressed in Chinese Hamster Ovary cell line.
Description
IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F. Members of this family demonstrate a structural motif termed a cysteine-knot that also characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links
Expression System
CHO
Species
Rat
Tag
Tag Free
Predicted N Terminal
Ala18 (IL-17A)&Arg29 (IL-17F)
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 15 kDa (IL-17A) & 15 kDa (IL-17F)
SDS-PAGE: 12-30 kDa, reducing conditions
Protein length
IL-17A (Ala18-Ser150, Ile46Leu) and IL-17F (Arg29-Ala161)
Bio-activity
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-18 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-18 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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