Recombinant Rat Il1b Protein, No Tag
Product Description
Cat
IMP-4603
Official Symbol
Il1b
Product Overview
Recombinant mature form of rat IL1B (Q63264-1) (Val 117-Ser 268) was expressed and purified.
Description
Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of a successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Expression System
E. coli
Species
Rat
Tag
No Tag
Predicted N Terminal
Met
Form
Lyophilized from sterile 40mM Tris, 0.15 M NaCl, pH 7.8, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The mature form of recombinant rat IL1B comprises 153 amino acids and predicts a molecular mass of 17.4 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions.
Protein length
Val117-Ser268
Endotoxin
Please contact us for more information.
Purity
> 98 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.1-0.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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