Recombinant Rat IL1R1 Protein (Leu20-Lys338), C-mFc-tagged

Product Description

Cat

IMP-10491

Official Symbol

Il1r1

Product Overview

Recombinant Rat IL-1 RI protein (Leu20-Lys338) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12.

Expression System

Mouse myeloma cell line

Species

Rat

Tag

C-mFc

Predicted N Terminal

Leu20

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 64.3 kDa (monomer)
SDS-PAGE: 85-100 kDa, reducing conditions

Protein length

Leu20-Lys338

Bio-activity

Measured by its ability to inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 5-20 μg/mL of rrIL-1 RI/Fc Chimera will inhibit 50% of the biological response due to 15 pg/mL of rrIL-1a.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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