Rat Il2 (NP_446288, 21 a.a.-155 a.a.) partial recombinant protein expressed in E. coli.
Expression System
E. coli
Species
Rat
Tag
Tag free
Form
Liquid, In PBS, pH 7.4 (10% glycerol).
Molecular Mass
15.6 kDa
Protein length
21-155
Endotoxin
< 1 EU/μg of protein (determined by LAL method).
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE
Storage
Store at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Bioactivity-ELISA 1
Il2 (Rat) Recombinant Protein stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 0.3 ng/mL.